P-57: Effects of Oral Administration of Gibberellic Acid Adult Male Rat on Serum LH,FSH, Testosterone and Testicular MDA

Background: Giberellic acid is a plant growth regulator,apentacyclicditerpene acid promoting growth and elongation of cells, and also a type of pesticide. In this study its oral administration effect on male rat hormones including gonadotropins (LH and FSH) and testosterone, and also lipid peroxidation product (Malondialdehyde) in testicular tissue was investigated. Materials and Methods: Twenty seven mature male Wistar rats were divided into 3 groups (each group n=9) control (no treatment), control- sham (1% methanol) and treatment group (gibberellic acid). Administrations were given with oral gavages every other day. The rats were sacrificed on 15th, 30th and 45th days of the experiment (3 rats for each day of each group), serum samples were taken and LH, FSH and Testosterone levels were measured with radioimmunoassay. Also testicular tissue was used for MDA measurement. Results: Serum FSH had no significant difference between the groups but serum LH had increased and Testosterone had decreased significantly (p<0.05) in the treatment group days 30 and 45. MDA in treatment group was higher than control and control- sham groups significantly. Conclusion: Giberellic acid can increase lipid peroxidation in testicular tissue and decrease testosterone, so it may interfere with normal fertility.